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凌笑梅

教授、博士生导师

凌笑梅  教授  博士生导师365bet娱乐在线副主任。

    话:010-82801590O);010-82805154L

    真:010-82802414

电子邮箱lingxm@bjmu.edu.cnpkulxm@126.com

通讯地址:北京市海淀区学院路38号,北京大学药学院药物分析教研室

    编:100191

多年来一直从事仪器分析和药物分析的教学和科研工作,主编了《高等仪器分析实验与技术》,并担任药物分析杂志编委、中国药学会药物分析委员会委员等。对色谱联用技术和高效毛细管电泳在医药领域中的应用做了大量的研究工作,主要研究工作有:(1)药物代谢动力学及代谢组学研究;(2)生物分子之间的相互作用及生物分子与药物分子之间相互作用研究;(3)天然产物及合成化合物中活性成分的定性定量筛选研究;(4)新药质量标准及稳定性研究;(5)手性药物拆分测定;(6)体内、体外脂肪酸含量测定等。发表相关学术论文70余篇。

主要研究方向

1、药物分析新技术和新方法研究及其在生物医药领域中的应用研究:采用毛细管电泳方法研究分子间相互作用研究,如生物大分子和小分子、生物大分子与生物大分子相互作用;手性物质的拆分及与生物大分子相互作用研究;毛细管色谱柱固定化技术研究(固定蛋白、细胞等)及在药物研究中的应用。

2、药物代谢动力学及代谢组学研究:采用现代仪器和色谱联用技术,进行药物体内的生物转化、血药浓度-时间曲线、吸收、分布、排泄、与血浆蛋白结合、对药物代谢酶活性影响和药物-药物相互作用及代谢组学研究等。

3、新药质量标准及稳定性研究:创新药物质量标准建立和稳定性研究。

主讲课程

1.定量分析          2.仪器分析          3.药物分析          4.高等仪器分析      5.毛细管电泳

6.生物药物分析      7.定量分析实验      8.仪器分析实验      9.药物分析实验      10.高等仪器分析实验

近几年代表性论文

1.     Jing Li, Wensi Lin, Weiwei Lin, Peng Xu, Jianmei Zhang, Haisong Yang, Xiaomei Ling, Metabolic profiling study on potential toxicity and immunotoxicity-biomarker discovery in rats treated with cyclophosphamide using HPLC-ESI-IT-TOF-MSBiomedical Chromatography, 2015, 29(5): 768-776

2.     Zhongjie Li, Yiran Zhao, Weiwei Li, Xiaobo Hu, MinYe, Xiaodan Zhang, Xiaomei Ling*Rapid screening and identification of active ingredients in licoriceextract interacting with V3 loop region of HIV-1 gp120 using ACE andCE-MSJournal of Pharmaceutical and  Biomedical Analysis, Journal of Pharmaceutical and Biomedical Analysis, 2015, 111: 28–35

3.     Peng Xu, MinLi, Yanping Βai, WeiLu, Xiaomei Ling*,WeidongLi, The effects of piracetam on heroin-induced CPP and neuronal apoptosis in ratsDrug and Alcohol Dependence, 2015.150: 141-146

4.     Zhongjie Li, Li Li, Xiaowei Wang, Junyi Liu, Xiaomei Ling*Interactions of carrageenan or its degradation with R15K by affinity capillary electrophoresisJournal of Chinese Pharmaceutical Sciences2015,246: 400-404

5.     Peng Xu, Yanping Βai, Haisong Yang, Jing Li, Wei Lu, Xiaomei Ling*High-performance liquid chromatography with fluorescence detection method for simultaneous quantitation of monoamine neurotransmitters and their metabolites in subregions of rat brain, Journal of Chinese Pharmaceutical Sciences2015,24(7):458-466

6.     Yiran Zhao, Zhongjie Li, Yong Jiang, Xiaodan Zhang,Xiaomei Ling*Rapid screening of anti-HIV ingredients in Artemisia rupestris L. extracts interacting with V3 loop region of HIV-1 gp120 and reverse transcriptase by affinity capillary electrophoresis and capillary zone electrophoresisJournal of Chinese Pharmaceutical Sciences2015,24(6):625-629

7.     Lu Yang, Pazilaiti.Yakufu, Meina Li, Xiaomei Ling*, Zhongjie Li, Ying Wang,Study on Interactions between Ligands (Compound A/22) and CCR4, ML40, 3 Extracellular Loops by ACE and CZEJournal of Chinese Pharmaceutical Sciences201423(3)186-189

8.     Meina Li,·Lei Lei,·Linghan Jia, Xiaomei Ling*, Jianmei Zhang, Yiran Zhao, Kewei Wang, Quantitative evaluation of interactions between Kv4.3 N-terminus and its auxilary subunit KchIPs by affinity capillary electrophoresis, Analytical Biochemistry, 2014,449:99-105

9.     Haisong Yang, Wensi Lin, Jianmei Zhang, Weiwei Lin, Peng Xu, Jing Li, Xiaomei Ling*Urine metabonomics analysis of the toxic effects of TM208 in rats by HPLC-IT-TOF/MS, Journal of Chromatography B, 2014, 959: 49-54

10.   Jing Li, Weiwei Lin, Dai Li, Yingfang Ao, Haisong Yang, Xiaomei Ling*A validated HPLC method for the determination of cefazolin sodium level in rabbit synovial fluid, Journal of Chinese Pharmaceutical Sciences 2014,23(9):649-653

11.   Pazilaiti.Yakufu, Hui Qi, Xiaomei Ling*, Wenjing Chen, Ying WangCCR4 Expressing Cell Cultured Adherently on a Capillary Wall as the Stationary Phase for Ligand Screening by ACE, Electrophoresis, 2013,344:531-540

12.   Wensi Lin, Jianmei zhang, Xiaomei Ling*, Ning Yu, Jing Li, Haisong yang, Runtao Li, Jingrong Cui, Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC-DAD: A cocktail approach, Journal of Chromatography B, 2013, 923-924: 29-36.

13.   Meina Li, Qing Wang, Xiaomei Ling*, Lu Yang, Zhongjie Li, Min Ye, Ying Wang,Interactions between N-terminal extracellular tail of CCR4 and natural products of licorice using capillary electrophoresis, Chromatographia, 2013, 76(13-14): 811-819.

14.   Wen-Jing Chen, Pazilaiti.Yakufu, Xiao-Mei Ling*, Hui Qi, Jun-Hai Xiao, Ying Wang,  N-terminal extracellular tail of CC chemokine receptor 4 (ML40) immobilized on capillary column to study the interactions between ML40 and its potential ligandsAnalytical Biochemistry, 2012,423(1): 1-6

15.   Jianmei Zhang, Wensi Lin, Xiaona Li, Ning Yu, Xiaomei Ling*, Ge Fu, Runtao Li, Jingrong Cui, Determination of 4-methylpiperaine-1-carbodithioc acid 3-cyano-3, 3-diphenylpropyl ester hydrochloride in Rats’ Plasma by Online-SPE-HPLC-DAD: Application to a Pharmacokinetic Study, Journal of Separation Science, 2012, 35(5-6): 721-725

16.   Xiao-na Li, Ning Yu, Jian-mei Zhang, Wen-si Lin, Xiaomei Ling*, Ge Fu, Runtao Li, Jingrong Cui, Determination of cyclophosphamide in rat plasma using on-line SPE HPLC-DAD, Journal of Chinese Pharmaceutical Sciences, 2012, 15(1): 38-44

17.   Peng Xu, Wensi Lin, Chuanjun Zhu, Kelin Liu, Ying Chang, Yuxin Cui, Xiaomei Ling*, Wei LuIdentification of dimethylamphetamine in a suspect exhibit, Journal of Chinese Pharmaceutical Sciences

18.   刘勇,袁丽佳,贺锐锐,韩南银,凌笑梅*. 一次性使用人体动脉血样采集器中二甲硅油含量的测定. 中国药学杂志[J],拟于2012年第16期发表

19.   徐鹏,李晓娜,刘克林,凌笑梅*,卢炜. 超快速液相色谱法测定新型“K2”香料中JWH-018的含量. 中国药物依赖性杂志[J]20124),282-284

20.   Shuyu Zhang, Hui Qi, Pazilaiti. Yakufu, Fang Zhao,  Xiaomei Ling(责任作者), Junhai Xiao, Ying Wang, Screening of thiourea derivatives and carbonyl-2-aminothiazole derivatives for potential CCR4 antagonists using capillary zone electrophoresis, Journal of Chromatography B,879 (2011) 75–82

21.   王皎, 李晓娜, 张建美, 凌笑梅*, 李润涛, 崔景荣,RP-HPLC法测定TM208的含量及有关物质,药物分析杂志,2011,311):99-102

22.   Wujie Li, Yinling Ma, Zhu Gan, Xiaomei Ling*, Zhenjun Yang, Qualitative and quantitative determination of oligonucleotide by non-gel capillary electrophoresisChromatographia(2011) 73:579–582

23.   Lu Pei, Lujia Xie, Qian Lin, Xiaomei Ling*, Zhu Guan, Zhenjun YangStudies on the adenosine deaminase-catalyzed conversion of adenosine and nucleoside prodrugs by different capillary electrophoresis modes, Analytical Biochemistry 414 (2011) 131–137

24.   Zhe Sun, Lin-Jie Tian, Qian Lin, Xiao-Mei Ling*, Hai-Jun Xiao, Ying Wang,Screening of chemokine receptor CCR4 antagonists by capillary zone electrophoresisJournal of Pharmaceutical Analysis2011,1(4): 264-269

25.   Xiaomei Jing, Yihao Bai, Xiaomei Ling*, Fangbin Han, Runtao Li, Jingrong Cui,Simultaneous determination of 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride and its major metabolite in rats by high-performance liquid chromatography, Journal of Chromatography Science, 2010,48: 125-129

26.   Wujie Li, Mou Cao, Lu Pei, Xiaomei Ling*, Bingchao Li and Zhenjun Yang, Study on steady-state kinetics of nucleotide analogues incorporation by non-gel capillary electrophoresis,Electrophoresis, 2010, 31: 507-511

27.   Wenjing Chen, Wujie Li, Xiaomei Ling*, Xiaowei Wang, Junyi Liu, Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis, Journal of Chromatography B, 2010, 878: 1714-1717

28.   Siting Wang, Yan Song, Jiao Wang, Xiaomei Ling*, Zemei Ge, Runtao Li and Jingrong Cui, Gas-fragmentation study of the novel synthetic zwitterionic drug 3-methyl-9-(2-oxa-2λ5-2H-1,3,2-oxazaphosphorine-2-cyclohexyl)-3,6,9-triazaspiro[5,5]undecane chloride (SLXM-2) by electrospray ionization tandem mass spectrometry, Rapid Communications in Mass Spectrometry, 2010, 24: 1181-1184

29.   Jiao Wang, Yan Song, Jianmei Zhang, Xiaona Li, Xiaomei Ling*, Runtao Liand Jingrong Cui, LC and LC–MS Study of the Bioavailability and Metabolites of TM208 after Oral and Intravenous Administration to Rat, Chromatographia, 2010, 72: 459-464

30.   王斯婷,李晓娜,王皎,凌笑梅*, 代谢组学及其分析技术,药物分析杂志,2010309):1792-1799

31.   Shuyu Zhang, Jun Cheng, Wenjing Chen, Xiaomei Ling*, Yiying Zhao, Jie Feng, Cheng Xiang, Hong Liang, Interactions Between Thrombin and Natural Products of Millettia nitita var. hirsutissima Using Capillary Zone Electrophoresis, Journal of Chromatography B,2009,877: 4107-4114

32.   Detao Kong, Siting Wang, Xiaomei Ling*, Runtao Li, Jingrong Cui, Determination of anti-tumor agent TM208, 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride, in rats by LC, Chromatographia, 2009, 70: 1721-1725

33.   Wenjing Chen , Ke Mou, Bo Xu, Xiaomei Ling*, Jingrong Cui, Ping Xu, Capillary electrophoresis for screening of 20S proteasome inhibitors, Analytical Biochemistry, 2009, 394(11): 62-67

34.   Zhe Sun, Xiaomei Ling*, Yingmei Zhang, Linjie Tian, Ying Wang, Interactions of the peptides from secreted human CKLF1 and the N-terminal extracellular tail of CCR4 analyzed by capillary zone electrophoresis, Chromatographia, 2009, 70(1/2): 287-292

35.   Wenjing Chen, Xiaomei Ling*, Advances of methods of protein immobilization on capillary, Chinese Journal of Analytical Chemistry, 2009, 37(6): 929–934.

36.   Yihao Bai, Zhen Chen, Xiaomei Ling*, Runtao Li, Jingrong Cui, Determination of Chloride 3-Methyl-9-(2-oxa-2k5-2H-1,3,2- Oxazaphosphorine-Cyclohexyl)-3,6,9- Triazaspiro [5.5] Undecane (SLXM-2) in Rats by LC:Application to a Pharmacokinetic Study, Chromatographia, 2009, 69(1-2): 111-115

37.   Shuyu Zhang, Ting Yin, Xiaomei Ling*, Hong Liang, Yuying Zhao, Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone, Electrophoresis, 2008, 29(16): 3391-3397

38.   Yi Liu, Shuyu Zhang, Xiaomei Ling*, Yuzhen Li, Yingmei Zhang, Wenling Han, Ying Wang, Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis, Journal of Peptide Science, 2008, 14(8): 984-988

39.   Yi Liu, Wujie Li, Xiaomei Ling*, Xianyin Lai, Yuzhen Li, Qingying Zhang, Yuying Zhao, Simultaneous Determination of the Active Ingredients in Abelmoschus manihot (L.) Medicus by CZE, Chromatographia, 2008, 67(9-10): 819-823

40.   Detao Kong, Xiaomei Ling*, Fangbin Han, Zemei Ge, and Jingrong Cui, Identification of the metabolite and cytochrome P450 isoforms involved in rat liver microsomal metabolism of TM208, Journal of Chinese Pharmaceutical Sciences, 2008, 17(1): 30-34.

41.   Zhe Sun, Xiaomei Ling*, Wei Sun, Junhai Xiao, Caihua Yin, Ying Wang,Studies on the interactions between lactam analogs and the N-terminal extracellular tail of CC chemokine receptor 4 by CZE (p NA), Electrophoresis, 2007, 28(17): 3064-3069

42.   Liu Yi,Xiaomei Ling*, Fan Hui, Zhong Yingcheng, Li Dan and Wang Ying. Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis,Journal of Chromatography A, 2007, 1143(1-2): 284-287.

43.   Xiaomei Jiang, Xiaomei Ling*, Fangbin Han, Runtao Li, Jingrong Cui, Analysis of 4-methyl-piperazine-1-carbodithioic acid 3-cyano-3,3- diphenyl-propyl ester hydrochloride and its major metabolites in rat plasma and tissues by LC-MS/MS,Journal of Separation Science,2007, 30(13): 2080-2085.

44.   Xiaomei Jiang, Xiaomei Ling*, Fangbin HanDetao Kong, Xiaoyan Liu, Runtao Li, Jingrong Cui, Studies on the metabolism of 4-methyl-piperazine-1- carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rat bile by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry, Rapid Communications in Mass Spectrometry.2007, 21(10): 1599-1605.

45.   Xiaomei Jiang, Xiaomei Ling*, Fangbin Han, Runtao Li and Jingrong Cui,Studies on the metabolism of 4-methyl-piperazine- 1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry, Journal of Pharmaceutical and Biomedical Analysis,2007, 44(5): 1127-1132.

46.   Xiaomei Ling*, Yi Liu, Hui Fan, Yingcheng Zhong, Dan Li and Ying Wang, Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis, Analytical and Bioanalytical Chemistry, 2007, 387(3): 909-916.

47.   Liu Yi, Shi Haiming, Sun Zhe, Xiaomei Ling*,and Tu Pengfei, Enantiomer separationof the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensisby capillary electrophoresis with HP-β-CD as chiral selector, Journal of Chromatography Science, 2007, 45(9): 605-609.

48.   凌笑梅*, 刘一, 赖先银, 张媛, 刘晓明, 屠鹏飞, 赵玉英, 崔景荣, 采用毛细管电泳方法以凝血酶为靶筛选天然药物提取化合物, 高等学校化学学报,2007, 28(2): 234-237.

49.   Yi Liu, Haiming Shi, Zhe Sun,Xiaomei Ling* and Pengfei Tu, Enantiomer separation of the four stereoisomers of 1-(4-hydroxy-3- methoxy)-phenyl-2- [4-(1,2,3-trihydroxy-propyl)-2-methoxy]-phenoxy-1,3-propandiol from Hydnocarpus annamensis by capillary zone electrophoresis with HP-β-CD as chiral selector, Chromatographia, 2006, 64(7-8): 483-486.

50.   Yi Liu, Xianyin Lai, Xiaomei Ling*, Yuying Zhao and Jingrong Cui, Interactions Between Thrombin with Flavonoids from Abelmoschus manihot (L.) Medicus by CZE, Chromatographia, 2006, 64(1-2): 45-50.

51.    Liu Yi, Zhang Yuan, Liu Xiaoming, Xiaomei Ling*Tu Pengfei, and Cui Jingrong, Interactions between thrombin and natural proudcts of Coreopsis tinctoria Nuttt. &Cistanche deserticola Ma. in capillary zone electrophoresis, Journal of Chinese Pharmaceutical Sciences, 2006, 15(1): 38-44.

52.    刘一,凌笑梅*,崔景荣. 毛细管电泳技术在研究生物分子间相互作用和药物筛选中的应用, 药物分析杂志,2006, 26(9): 1350-1357

53.    姜小梅,凌笑梅*,李润涛,崔景荣. 高效液相色谱-电喷雾离子阱质谱法分析大鼠体内4-甲基哌嗪-1-二硫代甲酸-(3-氰基-33-二苯基)丙酯盐酸盐及其主要代谢物,药学学报,200641(11):